Vitamin B12 Test


Vitamin B12 plays an essential part in red blood cell growth, cell metabolism, neuron function, DNA production – it’s also key during pregnancy to support the baby's development. Those following vegan and frequently vegetarian diets are most at risk of Vitamin B12 deficiency due to not meeting their requirements through animal sources such as meat, fish, and dairy products. However, supplementation can help to meet these needs.

While most individuals do receive enough of this Vitamin B12, it becomes more difficult to absorb this vitamin as people get older. B12 deficiency is common in those who have had weight loss surgery or another procedure that removed part of their stomach – a long history of alcohol consumption can also make it more likely.

Optimal B12 levels have been linked to benefits for (ICONS required):












What we measure


Methylmalonic acid (MMA) is a naturally occurring chemical produced at small levels during metabolism, and Vitamin B12 is necessary during this process to help your body convert food into energy. Without sufficient levels of Vitamin B12, your body produces more MMA. So, high MMA levels typically indicate a B12 deficiency. Having this deficiency can in turn cause significant health concerns, such as anemia, exhaustion and difficulties with the brain and spinal cord, and it is significantly linked to cognitive impairment, dementia, and Alzheimer's disease development too.


Our Vitamin B12 urine test detects the level of MMA, which can help reveal any specific signs of B12 deficiency. The more MMA you have based on this test, the more deficient your Vitamin B12 levels are likely to be. With this urine test, the results are delivered in millimoles of MMA per millimole of creatinine (cr). When the findings show less than 2.0 mmol MMA/mol cr, it indicates optimal Vitamin B12 status. A consequence of more than 3.8 mmol MMA/mol cr means that your B12 level is very low and should be confirmed with additional testing.


Monitoring MMA levels in the urine makes it simpler to detect the slightest elevations and offers more sensitive and efficient results than its blood test counterpart. Our Neuroptimax Diagnostics home-based urine testing kits make monitoring your health simple and convenient so you can get back to performing at your best.


Optimise Your Vitamin B12 Levels in 3 Easy Steps

1 Measure

You won’t know if your Vitamin B12 level is optimal just because you eat a balanced diet or take supplements — it must be measured.


2 Modify

Once you know your Vitamin B12 level, it may be optimised with simple dietary changes or may prompt other lifestyle changes.


3 Monitor

Testing your Vitamin B12 level every 4-6 months will ensure that your levels stay optimal.




Order: Choose the test service you would like. The sample collection kit typically arrives in 3 to 5 days.

Collect Sample: Follow the simple kit instructions to collect your urine sample from the comfort of home. Once you collect your sample, mail it back to our lab using the pre-paid envelope.

Get Results: In 2-4 weeks you will receive an email letting you know your personalised results are ready. You'll also get detailed advice with easy-to-understand graphics for your food, exercise, sleep, supplements, and much more. Take action and track your progress to take your health to new heights.



Why is the uMMA result reported as mmol/mol creatinine?

MMA can be measured in blood, but can also be measured in the urine, as long as we take into account how well the kidneys are working. We do this by measuring a substance called creatinine that is filtered into the urine by the kidneys. When we compare the urinary MMA to urinary creatinine, it improves the accuracy of the MMA test in urine when compared to blood, because it is not affected by mild to moderate decrease in kidney function.

Are the uMMA test results reported in units or just ‘high’ or ‘low’? 

The uMMA test results are reported in specific units. The units are mmol MMA/mol cr. Normal vitamin B12 status is indicated when the result is below 3.6 mmol MMA/mol cr, but new research shows that an optimal level is below 2.0 mmol MMA/mol cr. A result above 3.6 mmol MMA/mol cr indicates a possible B12 deficiency. More testing is often needed to confirm true deficiency, so please consult with your healthcare provider if you get this result.

Why has my doctor never recommended a Vitamin B12 uMMA test?

Doctors typically do measure vitamin B12 levels, and some may measure blood MMA levels. However, the urinary test for MMA is not used as much because it’s less common and may be more expensive.

Does the urine sample need to be collected at a specific time?

Short answer. Yes, take a sample of your first pee of the day. This helps reduce any “noise” in the sample.